WebOct 3, 2013 · A total of 29,861 pairs of reads (Supplemental Table S4) mapped to 25 elements in the constructed library, indicating the presence of transgene(s), such as the maize Ubiquitin promoter, CaMV35S ... WebMay 6, 2015 · The CaMV35S polyA module in the plasmid pCAMBIA-1300-221 was selected as the terminator for the basta-resistance cassette, and the entire sequence from the first base immediately after the hygromycin module to the unique SacII restriction site of the plasmid pCAMBIA-1300-221 was amplified with the primer pair CaMV35S-5 and …
Genetic Transformation of a Hepatoprotective Plant, …
Web而cDNA则是利用polyA尾使用polyT反转录出来的。 什么是 18srrna 答: 18SrDNA 是相对于基因组而言,18SrDNA 转录后即为 18SrRNA 。 18SrDNA在生物中是最为保守的基因之一,所以也常用来进行生物分类的依据。 WebJan 1, 2016 · Homeowners aggrieved by their homeowners associations (HOAs) often quickly notice when the Board of Directors of the HOA fails to follow its own rules, or … syncrophaser
Schematic representation of the expression modules in …
WebSchematic diagram of the T-DNA region of the binary vector pCAMBIA1391; LB: left border, RB: right border, polyA-CaMV: polyA signal, HygR: encoding hygromycin B phosphotransferase, 35S: CaMV35S enhance promoter, MCS: multiple cloning site, GUS: encoding β-glucuronidase, NOS-nopaline synthase terminator. (b). The GUS activity … WebJan 3, 2024 · The reporter beta-glucuronidase (GUS) gene was driven by the CaMV35S promoter and terminated by the CaMV35S polyA signal. Successful transformation was detected using polymerase chain reaction (PCR) technology. Detecting a positive result for the T-DNA hptII gene and a negative result for the intercistronic region between Vir B … Webby a CaMV35S promoter and terminated by a CaMV35S polyA; (B): same as ‘A’ except that the construct harbored a hygromycin phosphotransferase (hptII) gene. Figure 2. Half-strength (0.5X) MS-agar plates for selection of transgenic Arabidopsis thaliana L. plants. Plate A: wild type (WT) thai marysville wa