His60 ni superflow レジン 原理
WebbHis 60 Ni Superflow是一种高容量的树脂，并可提供高纯度的蛋白。. 该产品提供1 ml和5 ml两种预装柱类型，可在任意LC系统中对His标签蛋白进行纯化，例如AKTA或者FPLC，并可以使用注射器手动纯化。. 该树脂可以快速、简便、可重复地进行色谱分离，并且可以再 … WebbClontech’s His60 Ni Superflow Resin is a high-capacity Ni-IDA resin that has been optimized for the efficient purification of expressed his-tagged proteins from bacterial, …
His60 ni superflow レジン 原理
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Webb该产品结合了His60 Ni Superflow Resin产品优势与磁珠分离方法的优势。 当样品放置在磁珠分离器中时，磁珠中有磁性的微粒可以帮助我们快速方便地分离微小差异的蛋白。 该产品属于改良型的Ni-IDA树脂，相比于其他Ni-NTA或者Ni-IDA树脂而言，容量高、金属离子泄漏少，纯度高。 产品特点 ·简便快速地纯化His标签蛋白 ·采用改良型的His60 Ni 树脂，获 … WebbHis60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells.
WebbHis60 Ni Magnetic Beads Protocol-At-A-Glance (031716) www.clontech.com Clontech Laboratories, Inc. A Takara Bio Company Page 1 of 4 I. Introduction His60 Ni Magnetic … Webb大腸菌からのHisタグ融合タンパク質の抽出に Nickel レジン 高収量を実現するNickel レジン Capturem™ His-Tagged Purification 短時間の簡便な操作で高純度ヒスタグ融合タ …
http://cms.takara.co.kr/file/lsnb/45_36-39.pdf WebbThe selected protein gene was cloned into Escherichia coli TOP10 and expressed in Escherichia coli BL21 (DE3) pLysS. Recombinant protein was expressed using isopropyl-β-D-1-thiogalactopyranoside (IPTG) and induction conditions were optimized. Finally, it was purified with Ni-IDA resin (His60 Ni Superflow) and a Western Blot assay was …
WebbHis60 Ni resin is compatible with batch/gravity-flow applications, as well as with the major automated liquid chromatography systems or manual syringe processing. The resin …
WebbHis60 Ni Superflow Cartridges His-tagged protein purification on any LC system Up to 60 mg/ml of recombinant his-tagged protein (1 ml cartridge) Up to 300 mg/ml of recombinant his-tagged protein (5 ml cartridge) Low metal ion leakage in eluted his-tagged protein For purification under native or denaturing conditions legends popcorn planoWebbHis60 Ni Superflow™ Resin—Highest Capacity Advantages: • Highest binding capacity (60 mg/ml) • Purify proteins under native or denaturing conditions • Low leakage of Ni2+ions Applications: • Protein labeling • Immunization to produce antibodies • Animal studies Figure 1. legends pool hall portsmouth nhWebbHisタグタンパク質の精製原理. Hisタグをつけたタンパク質を大腸菌などで発現させ、Niがついているアフィニティー担体を用いて精製します。. 各社からHisタグタンパク … legend springs elementary calendarWebbHis60 Ni Superflow Cartridge Purification Kit His60 Ni Gravity Columns His60 Ni Gravity Column Purification Kit His60 Ni Buffer Set His60 Ni Magnetic Beads 6×His mAb-HRP Conjugate 6×His Monoclonal Antibody (Albumin Free) 6×HN Polyclonal Antibody Magnetic Stand Capturem His-Tagged legends premium experiences companyWebbHis60 Ni Superflow Cartridge Purification Kit (5 x 1 ml) provides 5 prepacked . His60 Ni Superflow Cartridges (each containing 1 ml of resin), as well as all the buffers needed … legends pompano beachlegends popcorn couponWebbHis60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the efficient purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. His60 Ni Resin is compatible with batch/gravity flow applications, as well as with the major automated liquid chromatography systems and manual syringe processing—and it … legend sportfishing